Amoeba ord stock

Stock cultures of amoeba. References In spite of this, it has attracted relatively little attention and is used by only a few groups of devoted cell biologists. A new class of uncoupling agents-carbonylcyanide phenylhydrazones CCP. Download citation. Inhibitors, the extremes of pH, and low temperature do not affect the observed uptake during the first few minutes of treatment, but after this short initial period the uptake decreases markedly or completely stops.

Publisher Summary This chapter discusses the potential of in vivo mitochondrial form and functional relationships in toxicology and pathology. Mitochondrial Polymorphism in Amoeba proteus. In: Jeon KW ed The biology of amoeba. While enucleating amoebae in previous studies, we found that if a very small portion of a nucleus was pushed through the membrane and exposed to the external medium, the amoeba expelled such a nucleus on its own accord. Providing treatments were terminated before cell viability was lost, all mitochondrial abnormalities were reversible on return to normal culturing conditions. Contracted mitochondria with dark matrix and wide cristae Type I , and expanded mitochondria with light matrix and narrower cristae Type II coexist in normal active amoebae, but their numbers can be varied according to different cell activity states. Tetrahymena are added to cultures at each feeding in numbers proportional to the number of amebae present.

J Cell Sci , , 01 Oct In our study the explanted rat embryo model demonstrated that in the head-fold region hyperglycemia produced morphologic alterations of mitochondria but no difference in the number of mitochondria per cell. Observations on mitochondrial staining for succinic dehydrogenase. J Reiss. Lester Packer John M. Variations de mitochondries dans des racines de Lycopersicum esculentum isolees, culture'es in vitro et sonmises a divers traitments: anoxie prolonge'e, agent de'couplant, plasmolyse.

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The condensed configuration corresponded to the configuration exhibited by freshly isolated resting mitochondria suspended in about 0. Similar stock images. In the present study, the role of the nucleus in this response was tested by injecting heterologous cytoplasm from Pelomyxa carolinensis into enucleate and enucleate Amoeba discoides. Summary The fine structure of the mitochondria ofAmoeba proteus was studied utilizing several methods of fixation. The mechanisms s of diabetic teratologic development has yet to be defined. Detailed consideration of the fact that two morphologically different types of mitochondria are present in the cytoplasm of Ch.
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Modifications caused by uncoupling agents. Microinjection of adenine nucleotides and substrates into the cytoplasm of Amoeba proteus followed by EM examination has been used in an attempt to relate alterations in mitochondrial morphology with functional changes. In respiratory steady state 1 and 4 the mitochondria showed an expanded configuration; in state 2, 3, 5, and uncoupled, they exhibited a condensed configuration. Amoebae were cultured, cloned, and studied using light- and electron-microscopic techniques. Robert A. Biochemistry, N.
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After completion of cytokinesis I the total number of mitochondria ca. D , Reversible ultrastructural changes were found to accompany change in metabolic steady states. Learn more. Reprints and Permissions.
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Its increasing popularity is reflected by a steadily rising impact factor. Ord MJ The viability of the anucleate cytoplasm of Amoeba proteus. Numerical, morphological and topographical heterogeneity of the chondriome during the vegetative life cycle of Polytoma papillatum. The journal reflects these aims. Charles J. Brent Finley Stata Norton. Search stock photos by tags bookauthorlilliefrankrat , booksubjectbiology , booksubjectzoology.
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The response to proteolytic enzymes. Mitochondrial ultrastructural transformations have been examined in intact eggs and embryos from three sea urchins, Arbacia punctulata, Strongylocentrotus purpuratus, and Lytechinus pictus. Great advances have been made in recent years in the use of ultrastructural staining methods for the localization within the mitochondria of specific enzymes, ionic species, and glycoproteins. Rehn Eugene C. Charles J. Comandon P.
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